ABSTRACT

Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques-such as labeling by immunofluorescence and fluorescent proteins-which have revolutionized cell biology. Quantitat

chapter 1|20 pages

- The Light Microscope

chapter 2|14 pages

- Optical Contrasting Techniques

chapter 3|14 pages

- Fluorescence and Fluorescence Microscopy

chapter 4|14 pages

- Image Capture

chapter 5|20 pages

- The Confocal Microscope

chapter 6|10 pages

- The Digital Image

chapter 7|16 pages

- Aberrations and Their Consequences

chapter 8|16 pages

- Nonlinear Microscopy

chapter 9|12 pages

- High-Speed Confocal Microscopy

chapter 10|14 pages

- Deconvolution and Image Processing

chapter 12|12 pages

- Green Fluorescent Protein

chapter 13|10 pages

- Fluorescent Staining

chapter 14|16 pages

- Quantitative Fluorescence

chapter 16|10 pages

- Evanescent Wave Microscopy

chapter 17|18 pages

- Beyond the Diffraction Limit