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Front MatterAbstract - Hi-Res PDF (163 KB) - PDF w/links (164 KB)
Chapter 1. Expression and Purification of G-Protein α Sub units in Escherichia coliMaurine E. LinderAbstract - Hi-Res PDF (477 KB) - PDF w/links (383 KB)
Chapter 2. Purification of Recombinant G Protein alpha and Beta-gamma Subunits from Sf9 CellsTohru KozasaAbstract - Hi-Res PDF (1290 KB) - PDF w/links (368 KB)
Chapter 3. Expresion and Functional Analysis of G Protein alpha Subunits in Mammalian CellsCatherine H. BerlotAbstract - Hi-Res PDF (729 KB) - PDF w/links (394 KB)
Chapter 4. Identification of Regulators and Targets of G Protein alpha Subunits Using the Yeast Two-Hybrid SystemJennifer L. Glick, Thomas E. Meigs, Patrick J. CaseyAbstract - Hi-Res PDF (1348 KB) - PDF w/links (347 KB)
Chapter 5. Functional Analysis of G Protein Beta-gamma SubunitsN. Gautam, A. N. Pronin, K. Yan, O. G. KisselevAbstract - Hi-Res PDF (1482 KB) - PDF w/links (518 KB)
Chapter 6. Immunological Techniques of G Protein AnalysisCatherine A. ChenAbstract - Hi-Res PDF (818 KB) - PDF w/links (431 KB)
Chapter 7. GTPase-Activating Proteins (GAPs) for Heterotrimeric G ProteinsElliott M. Ross, Yaping Tu, Jun Wang, Gloria H. Biddlecome, Peter Chidiac, Suchetana MukhopadhyayAbstract - Hi-Res PDF (353 KB) - PDF w/links (267 KB)
Chapter 8. Fatty Acid Acylation of G protein alpha SubunitsPhilip B. WedegaertnerAbstract - Hi-Res PDF (849 KB) - PDF w/links (447 KB)
Chapter 9. ADP-Ribosylation of G Protein with Cholera Toxin or Pertussis ToxinMarilyn J. WoolkalisAbstract - Hi-Res PDF (1920 KB) - PDF w/links (493 KB)
Chapter 10. Analysis of Receptor-G Protein Coupling With Pertussis Toxin-Resistant Mutant of Gi and GoRonald Taussig, Albrecht MoritzAbstract - Hi-Res PDF (1352 KB) - PDF w/links (418 KB)
Chapter 11. Microinjection of Antibodies Neutralizing G Protein FunctionVickie J. LaMorte, Judy L. Meinkoth, James R. FeramiscoAbstract - Hi-Res PDF (2054 KB) - PDF w/links (357 KB)
Chapter 12. Agonist-Promotes [35S] GTP-yS-Binding as a Probe of Receptor G Protein Communication in Reconstituted Sf9 CellsAlastair J. BarrAbstract - Hi-Res PDF (952 KB) - PDF w/links (438 KB)
Chapter 13. Methods of Sequestering Beta-gamma in the Intact CellGregory J. Della Rocca, Walter J. Koch, Robert J. LefkowitzAbstract - Hi-Res PDF (868 KB) - PDF w/links (421 KB)
Chapter 14. In Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling Via DNA/RNA Antisense StrategiesZhiyong Cheng, Xunxian Liu, Hsien-yu Wang, Craig C. Malbon, Hui Ling LiAbstract - Hi-Res PDF (1753 KB) - PDF w/links (455 KB)