ABSTRACT

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization

chapter 1|46 pages

Probes

chapter 2|18 pages

Principles of Methodology

chapter 3|22 pages

Sample Preparation

chapter 4|64 pages

Post-Embedding Technique

chapter 5|56 pages

Pre-Embedding Technique

chapter 6|46 pages

Frozen Tissue Technique

chapter 7|48 pages

Semithin Sections

chapter 8|36 pages

Controls and Problems

chapter |52 pages

Examples of Results