ABSTRACT
Chromatographic methods are being used routinely for chemical analysis since 1950s, and for automated analysis of process streams in process
control (process chromatography). The uses of chromatographic methods for analysis are well known and are being used for a variety of cases. As for example, a high-performance liquid-chromatographic procedure for 5-hydroxy-3-indoleacetic acid is described and compared with a colorimetric method in which 1-nitroso-2-naphthol is used [1]. A simple and cost-effective assay for urinary 5-hydroxyindole acetic acid (5-HIAA) is reported [2]. Chromatographic separation was used for separation of Uroporphyrins I and III on a microporasil column using n-heptane, glacial acetic acid, acetone and water [3]. A number of examples are available where chromatographic methods were used for the fractionation and separation for compounds of similar or closely related physical properties [4, 5]. Off late chromatographic separation has become one of the main tools for separation of racemic mixtures to obtain chiral drugs containing only one enantiomer [6, 7]. The effects of adsorption characteristics of a displacer on the displacement efficiency of desorption chromatography were also reported [8].
